Development of a polymerase chain reaction/restriction fragment length polymorphism method for Saccharomyces cerevisiae and Saccharomyces bayanus identification in enology
نویسندگان
چکیده
منابع مشابه
CLONING AND EXPRESSION OF HUMAN IFNα2B GENE IN SACCHAROMYCES CEREVISIAE
Interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. Recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (HCL). The aim of the present study is to apply yeast strain for secreting human IFNα2b fol...
متن کاملPig species identification in meatballs using polymerase chain reaction- restriction fragment length polymorphism for Halal authentication
The detailed information on the chemical and nutritional content is essential for consumers in choosing meat-derived food products. For moslem communities, it is prohibited to consume pork-contained or other pig derivatives foods. Unfortunately, meat adulteration by means of mixing beef and chicken with pork or other pig derivatives frequently occurs in the market. This habits cause difficult i...
متن کاملWhole-blood polymerase chain reaction and restriction fragment length polymorphism: a simplified method by microwave irradiation.
OBJECTIVES The aim of the present study was to develop a simple, quick and cheap method to process whole-blood samples for the molecular techniques polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) without the use of expensive reagents or sophisticated machines. MATERIALS AND METHODS Venous whole-blood samples were collected from 40 individuals. The samples w...
متن کاملIdentification of fungemia agents using the polymerase chain reaction and restriction fragment length polymorphism analysis.
Prompt and specific identification of fungemia agents is important in order to define clinical treatment. However, in most cases conventional culture identification can be considered to be time-consuming and not without errors. The aim of the present study was to identify the following fungemia agents: Candida albicans, Candida parapsilosis, Candida tropicalis, Candida glabrata, Cryptococcus ne...
متن کاملMolecular identification of Giardia duodenalis in Ecuador by polymerase chain reaction-restriction fragment length polymorphism
The aim of this study was to determine the genetic diversity of Giardia duodenalis present in a human population living in a northern Ecuadorian rain forest. All Giardia positive samples (based on an ELISA assay) were analysed using a semi-nested polymerase chain reaction-restriction fragment length polymorphism assay that targets the glutamate dehydrogenase (gdh) gene; those amplified were sub...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1996
ISSN: 0378-1097
DOI: 10.1016/0378-1097(96)00117-6